Promega Corporation

Diamond™ Nucleic Acid Dye

Diamond™ Nucleic Acid Dye is a sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels. Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system, such as by UV transillumination with a Polaroid® or digital camera, GE ImageQuant™ or Bio-Rad Gel Doc™ systems.

The concentrated dye is stable for up to 90 days at room temperature. Diamond™ Nucleic Acid Dye does not require prewashing or destaining of gel...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega UK Ltd only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Diamond™ Nucleic Acid Dye

Close Window

Diamond™ Nucleic Acid Dye

 Components

  • Diamond™ Nucleic Acid Dye

    H118A1 x 500μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500μl
- H1181 £ 57.00 Add to cart


You Also May Be Interested In

Storage Conditions

Store at room temperature (22–25°C) for up to 90 days. Store at –20°C for long-term storage.

For product intended use please see Patents & Disclaimers tab.

Overview of the Diamond™ Nucleic Acid Dye protocol.Figure 1. Overview of the Diamond™ Nucleic Acid Dye protocol.
Diamond™ Gel Nucleic Acid staining of DNA separated on a 1.2% Clear E-gel®.Figure 2. Diamond™ Gel Nucleic Acid staining of DNA separated on a 1.2% Clear E-gel®.

Ten microliters of BenchTop 1kb DNA Ladder (Cat.# G7541) was loaded into lane 1. Twofold serial dilutions of the ladder were prepared in 1X Blue/Orange Loading Dye (Cat.# G1881) and loaded into lanes 2–12. Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged using Molecular Imager® Gel Doc™ XR+ System with Image Lab™ Software (Bio-Rad) using the SYBR® Gold application.

Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.Figure 3. Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.

Ten microliters of BenchTop 1kb DNA Ladder (Cat.# G7541) was loaded into lane 1. Twofold serial dilutions of the ladder were prepared in 1X Blue/Orange Loading Dye (Cat.# G1881) and loaded into lanes 2–10. Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged using Molecular Imager® Gel Doc™ XR+ System with Image Lab™ Software (Bio-Rad) using the SYBR® Gold application.

Use Restrictions

H1181 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

H1181 Patent Pending.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.