Biochemical Buffers and Reagents

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Diamond™ Nucleic Acid Dye provides a robust method for staining many gel types, along with the convenience of room temperature stability. It's more sensitive than ethidium bromide, so you use less sample and ladders for visualisation, saving you money with every gel you run.

Comparable in performance to SYBR^® Gold, it is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system.

Diamond™ Nucleic Acid Dye does not require prewashing or destaining of gels.

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Acrylamide, Molecular Grade

Resolve very small DNA fragments, such as those generated by sequencing reactions, using polyacrylamide gel electrophoresis.

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Agarose LMP

Preparative Grade for Small Fragments: Premium, DNase/RNase-free agarose used for isolation of DNA fragments 10-1,000bp. Isolated fragments can be used in many downstream applications.

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IPTG, Dioxane-Free

IPTG induces β-galactosidase activity by binding to and inhibiting the lac repressor, allowing you to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the lacZ or lacZ α-peptide gene.

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X-Gal

X-Gal: Used in conjunction with IPTG to detect β-galactosidase activity. Tested for use with the pGEM®-Z vectors in chromogenicity assays.

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This Protocols and Applications Guide chapter provides basic information on preparing buffers for biochemical reactions.