Notes: To identify molecules that affect metastasis signaling pathways downstream of HER2-Y1248 phosphorylation, suppression subtractive hybridization assays (SSH) were
performed using MDA-MB-468 cells overexpressing HER2 and control MDA-MB-468 cells expressing HER2 without the Y1248 phosphorylation site. Reactions were cloned
using a T-vector system, transformed and plated. Positive clones from each assay were selected and grown overnight in 2ml deep-well plates. The Wizard^® Magnesil^® Plasmid Purification System was used to isolate plasmids for BigDye™ sequencing. (4134)

Notes: Eight Pennisetum squamulatum and seven buffelgrass BACs containing the apospory-specific genomic region (ASGR) marker ugt197 were randomly sheared to 1.5–3.0kb in size. The fragments were blunt-ended, size fractionated on a gel and the appropriate fraction excised and ligated into a plasmid. Random transformants were grown in deep-well plates and incubated for 18 hours. Plasmid DNA was isolated using the Wizard^® MagneSil^® Plasmid DNA Purification System on a Biomek^® 2000 workstation. The purified plasmid was sequenced with BigDye^® reaction mix using 150–300ng DNA. (3448)

Notes: Suppression subtractive hybridization (SSH) was performed on six breast cancer tumors. The PCR products were T/A cloned, grown and isolated using the Wizard^® MagneSil^® Plasmid Purification System. The purified plasmid DNA was then sequenced using BigDye^® 3.0 Sequencing Mix and the ABI 3700 Genetic Analyzer. (3446)

Notes: Methods to align chromosome structure were explored by the authors of this study. A BAC library of sorghum, a commercially important cereal crop, was mapped to define a contig covering chromosome 3.  Each BAC was subcloned by digesting with EcoR I and Xho I and ligating into a plasmid vector. After transformation into DH5α ™ cells, 16-96 colonies were picked and grown up in 96-well, deep-well plates containing 1.4ml LB medium/well. Overnight cultures were used for plasmid purification using the MagneSil^® Plasmid Purification System and a Beckman Coulter Biomek^® 2000 automated laboratory workstation. The resulting plasmids were sequenced with plasmid-specific primers using Applied Biosystem's BigDye™ v2.0 chemistry. The resulting sequence was compared to the complete rice genome to map similarities.  (2763)