Nano-Glo® In-Gel Detection System Technical Manual
Instructions for Use of Product(s)
N3020
Literature # TM527
The Nano-Glo® In-Gel Detection System directly detects the luminescence of NanoLuc® fusion proteins in a polyacrylamide gel matrix, following protein separation by polyacrylamide gel electrophoresis (PAGE) under either native or denaturing SDS conditions (native PAGE or SDS-PAGE). Compared to Western blotting (immunoblotting), the Nano-Glo® In-Gel Detection System offers superior speed, sensitivity and specificity with a simple protocol that does not require transfer to membranes, eliminating the need for blocking or antibodies. Following native PAGE, NanoLuc® activity is maintained, so the gels can be placed directly into Nano-Glo® In-Gel Detection Reagent and imaged. Following SDS-PAGE, the denaturing gels are first washed with isopropanol and water to remove the SDS detergent and allow protein refolding prior to adding detection reagent and imaging the gel.
Printed in the USA 10/17.