Ribonuclease H
Endonuclease that Specifically Hydrolyzes the Phosphodiester Bonds of RNA Hybridized to DNA
- Removes the RNA strand prior to second-strand cDNA synthesis
- Analyzes in vitro polyadenylation reaction products
- Supplied at a concentration of 0.5–2u/μl
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
Catalog Number: M4281
Catalog Number: M4285
Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3′-OH and 5′-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. This endonuclease can be used in applications such as microarrays.
Reference
- Goodwin, E.C. and Rottman, F.M. (1992) Nucl. Acids Res. 20, 916.
Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 50% (v/v) glycerol, 0.2mg/ml BSA.
Source: Recombinant E. coli strain.
QC Tests: Activity, DNase, RNase, endonuclease, cDNA synthesis.
Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
RNase H |
M428A | 1 × 50u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
RNase H |
M428C | 1 × 250u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
Resources
No related resources available
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