PKA Kinase Enzyme System

Kinase Enzyme Systems for Detecting Kinase Activity
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Easily Screen and Profile PKA Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V4246

$ 545.00
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PKA Kinase Enzyme System
2,500 units
$ 545.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKA Kinase Enzyme System contains:

  • PKA Kinase, 2,500 units (Bovine, recombinant full-length). MW: ~40kDa.
  • Kemptide (LRRASLG) Substrate.
  • Reaction Buffer, DTT.

Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. Intracellular targets include ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.

PKA NCBI Database Entry.

The PKA Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Specifications

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What's in the box?

Item Part # Size Concentration Available Separately

DTT

P117A 1 × 100μl 100mM View Product

5X Reaction Buffer A

V307C 1 × 1.5ml

cAMP-Dependent Protein Kinase, Catalytic Subunit

V516A 1 × 2,500u

Kemptide

V560A 1 × 1mg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V4247 Change Configuration

What's in the box?

Item Part # Size

PKA Kinase Enzyme System

V4246 1 × 2,500 units

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

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