Dr Gary Allenby on Kinase Target Engagement in Live Cells in 3D Micro-scaffolds
The ability to measure binding affinity and drug-target residence time provides crucial information during the drug discovery process. In this short video, Dr Gary Allenby (Chief Scientific Officer at Aurelia Bioscience Ltd) talks about his experience with NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay. By employing this live-cell energy transfer technology and 3D micro-scaffolds, Gary is able to perform quantitative analysis of intracellular kinase target engagement.
NanoBRET™ Target Engagement (TE) Technology is the first one to allow assessment of the kinase inhibitors’ potency and selectivity in living cells. Unlike the traditional techniques, it measures under physiological pH and physiological ATP levels.
To support kinase biology, Promega has just launched more than 100 individual full-length kinase vectors. The vectors contain either N-terminal or C-terminal fusions of the kinase of interest and NanoLuc® luciferase. Browse here ›
Read more about Aurelia Bioscience’s kinase screening ›
Learn more about NanoBRET™ TE Technology &rsaquo