HLA Typing at Anthony Nolan Research Institute:
Promega’s Products Supporting
a Long-read Next Generation Sequencing Workflow
The Anthony Nolan Research Institute is a part of Anthony Nolan, a UK charity that maintains
a register of volunteers willing to donate their blood stem cells to patients with blood cancer,
and other haematological disorders, as well as improving the outcome of bone marrow transplantation. The Institute is solving the three main problems in transplantation:
graft-versus-host disease (GvHD), which is caused by differences in human leucocyte antigens (HLA); viral infection; and the separation of GvHD from the graft-versus-leukaemia (GvL) effect.
The Institute’s scientists currently perform HLA typing on DNA samples from blood, cell lines or buccal swabs. They use polymerase chain reaction (PCR) with DNA barcoded primers which allows them to multiplex both samples and loci into a single sequencing reaction. They generate the HLA sequences using PacBio Single Molecule, Real-Time (SMRT) Sequencing.
Dr Neema Mayor (Head of Immunogenetics Research) and Dr Thomas Turner
(Senior Postdoctoral Research Scientists) discuss the important role of the Anthony Nolan Research Institute in matching blood stem cells from donors with potential recipient patients.
In this fascinating video, Promega’s flagship products are shown to play an integral part in supporting long-read sequencing and HLA typing to provide the highest resolution match.
Maxwell® ProNex® QuantusTM Fluorometer GoTaq® Long PCR Master Mix