Vânia Costa is a Senior Strategic Field Applications Scientist at Oxford Nanopore Technologies. She has been part of the company for over eight years and has a strong background on extractions and sample preparation. She is part of the Technical Services team, and her role is focused on supporting high-throughput projects, from planning until completion. Previously to her current role, she was part of the Applications team where she focused on testing and optimising extractions on a variety of sample types for downstream analysis with nanopore devices. 

Oxford Nanopore sequencing with Promega Maxwell

Research human blood samples were used to assess the performance of three Promega extractions kits on the Maxwell device with Nanopore sequencing. The aim was to compare extracts from the different kits while determining the effect of adding some sample manipulation, namely fragmentation. In addition to this, stability of the extracts at -20C for one year was also evaluated.

We observed that whilst not all kits perform the same, and some level of fragmentation is beneficial, Promega Maxwell extractions are compatible with Nanopore sequencing. Regarding stability, we can confirm that the extracts remain stable after one year with little degradation observed, and with very similar performance to the fresh extractions. 

We will present the results of the comparison between the different kits, with and without sample manipulation, throughout time, and provide useful information on how to work with Promega extracts to obtain the best performance with Nanopore Sequencing.

Coming soon

Coming soon