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Notes: Epicatechin gallate, a catechin derivate from green tea, was determined to induce apoptosis in human squamous carcinoma (HSC-2) cells but not in normal human gingival (HGF-2) fibroblasts. The CaspACE™ Assay System, Colorimetric, was used to assess caspase-3 activities in HSC-2 cells that had been exposed to 0-250uM epicatechin gallate in culture. (3241)

Notes: The CaspACE™ Assay System was used to determine in situ caspase acitiviy. (2421)

Notes: Promega’s Human Recombinant Tumor Necrosis Factor-α (rhTNF-α) was added to bovine peripheral blood neutrophils (PMN) to induce expression of lymphocyte function-associated antigen 1 (LFA-1). Researchers incubated 1 x 10⁶ cultured bovine PMNs with 50ng of rhTNF-α for 15 or 60 minutes.  LFA-1 was detected by flow cytometry. The authors also used the Colorimetric CaspACE™ Assay System to assess apoptosis in bovine PMNs. For these studies, protein normalized cell lysates were used to compare caspase-3 activity to LFA-1 expression. (2699)

Notes: Tumore necrosis factor α-induced apoptosis of permanently transfected HeLa cells expressing the IG20 cDNA or its splice isoforms were assayed using the CaspACE™ FITC-VAD-FMK In Situ Marker. The activation os specific caspases was determined using the CaspACE™ Assay System. The Dual-Luciferase Assay System was used to measure TNF α-induced NF-κB activation in HeLA-IG20 cells. (2418)

Notes: Spontaneously immortalized granulosa cells were incubated with 50μM of the pan-caspase inhibitor Z-VAD-FMK to demonstrate that apoptosis occurs after serum withdrawal or EGTA treatment. The inhibitor was added after treatment of the cells for 5 hours. Researchers also used Promega's Colorimetric CaspACE™ Assay System to measure caspase-3 activity in cell lysates. Results were expressed as fold increase in activity.  (2669)

Notes: The CaspACE™ Assay System, Colorimetric, was used to measure caspase 3 activity in lysates of MOD mouse mammary carcinoma cells stably transfected with a temperature-sensitive p53. The cells were incubated with and without 50µM Z-VAD-FMK Inhibitor at 30°C for up to 18 hours prior to lysis and assay. The caspase 3 activity was at a maximum 12 hours after the 30°C step (i.e., the p53 is induced) in the temperature-sensitive p53 cells. (1099)

Notes: The CaspACE™ Assay System, Colorimetric was used to analyze DEVDase activity in rat cardiomyocytes exposed to various compounds under hypoxic conditions. The DEVDase activity was measured with the pan-caspase inhibitor Z-VAD-FMK as well as a caspase-3-specific inhibitor, Ac-DMOD-CHO. The specific activity is reported. (2057)

Notes: The CaspACE™ Assay System, Colorimetric, was used to measure caspace-3 like protease activity in A7r5 vascular smooth muscle cells transfected with AT2 receptor. (2129)

Notes: L929 cells were transfected with Hsp90, selected, and treated with 1 µM staurosporine for 18 hours. Caspase-3 activity was measured using the CaspACE™ Assay System, Colorimetric. (2130)

Notes: The CaspACE™ Assay System was used to analyze caspase-3 activation of the mouse liver homogenates derived from mice infected with adenoviruses expressing either β-galactosidase or IκBα. Adenovirus infection activates NF-κB, which in turn increases Fas expression and eventually leads to increased apoptosis. The IκBα delayed the onset of apoptosis in the 2- to 8-hour range. (It is not clear whether the authors used the CaspACE™ Assay System, Fluorometric, or CaspACE™ Assay System, Colorimetric, by the description in the paper). (0857)