Imaging Apoptosis in Living repTOP Reporter Mice Scientific Poster

Abstract

VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) is a firefly luciferase prosubstrate containing the DEVD tetrapeptide sequence recognized by caspase-3 and -7. Upon activation of caspase-3 or -7, the DEVD peptide is cleaved, and the liberated aminoluciferin reacts with luciferase.

VivoGlo™ Caspase-3/7 Substrate was designed to study the apoptotic pathway in vivo in mice. It has a minimum solubility of 500mg/ml in PBS, and the resulting solution is stable for at least 3 days at room temperature. Injection is usually done via the intraperitoneal route and imaging is generally started 10 minutes after injection.

These two novel technologies, repTOP™ mice and VivoGlo™ Caspase- 3/7 Substrate make it possible, for the first time, to see where and when Caspase-3/7 is active in living mice.