pNL3.2.CMV Vector

Instructions for Use of Product(s)
N1411

Literature # 9PIN141

The pNL3.2.CMV Vector is designed for constitutive expression of the NanoLuc® reporter fused to a PEST destabilization domain (NlucP). The NlucP fusion protein naturally accumulates at low intracellular levels due to constitutive proteosomal degradation, thus serving as a negative control for experiments configured to measure regulated changes in NanoLuc® luciferase expression levels. This vector also encodes a hygromycin resistance gene for selection in mammalian cells and an ampicillin resistance gene for selection in bacteria.

Revised 9/16