pFN10A (ACT) Flexi® Vector

Instructions for Use of Product(s)
C9331

Literature # 9PIC933

The pFN10A (ACT) Flexi® Vector is designed for use with the CheckMate™ /Flexi® Vector Mammalian Two-Hybrid System (Cat.# C9360), the Flexi® System, Entry/Transfer (Cat.# C8640 )and the Flexi® System, Transfer (Cat.# C8820). The pFN10A (ACT) Flexi® Vector contains a herpes simplex virus VP16 activation domain upstream and in-frame of the cloning site. The pFN10A (ACT) Flexi® Vector also contains a neomycin resistance gene under the control of the SV40 promoter for long-term selection of transfected mammalian cells. Transfection of a pFN10A (ACT) Flexi® Vector containing a cloned protein coding region into mammalian cells provides resistance to the antibiotic G-418 (Cat.# V7983 or V8091) and allows selection of transfectants. In common with other Flexi® Vectors, the pFN10A (ACT) Flexi® Vector contains a CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression, a T7 promoter for in vitro expression of the protein-coding region, a barnase gene for positive selection of the insert, an ampicillin resistance gene for selection of the plasmid, and unique SgfI and PmeI sites that allow easy insertion and transfer of the insert of interest. Do not use the pFN10A (ACT) Flexi® Vector without an insert as a negative control because the presence of the barnase gene decreases the viability of the transfected cells. The pFN10A (ACT) Flexi® Vector containing a cloned protein-coding region can be used to transfer the insert to other Flexi® Vectors with different expression options using the Flexi® Systems. For more information, see the CheckMate™/Flexi® Vector Mammalian Two-Hybrid System Technical Manual #TM283 and the Flexi ® Vector Systems Technical Manual #TM254.

Printed in USA. Revised 10/16.