The TUNEL assay, while popular for the detection of apoptotic cells, can yield highly variable results when used for apoptosis detection on research samples from various disease states. Use of a second, more specific marker of apoptosis, such as Promega's Anti-PARP p85 Fragment pAb, significantly enhances identification of apoptosis in pathology research specimens.
Promega Notes 72, 7.
Michiel Knaapen, Martine De Bie, Johannes Muhring and Mark Kockx