Previously, we described a strategy to construct stable cell
lines expressing two luciferases on two plasmids for a GPCR reporter assay. In
this report, we further demonstrate the utilities and advantages of the
dual-reporter luciferase GPCR assay through additional examples including an
agonist screen of a LOPAC compound collection.
Cell Notes 16, 22–25.
Aileen Paguio, Denise Garvin, Brad Swanson, Tracy Worzella, Keith Wood, and Frank Fan