These vectors are designed for use with Promega cell-free expression systems, including rabbit reticulocyte lysate, wheat germ extract, and insect cell extract-based systems.
Expression in Insect Cell Lysates
The pF25A and pF25K ICE T7 Flexi® Vectors are designed for maximal protein expression in the TNT® T7 Insect Cell Extract Protein Expression System.
Expression in Reticulocyte Lysate
The pTNT™ and pCMVTNT™ Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6 or T7-based coupled in vitro transcription/translation systems.
Vectors for Wheat Germ Systems
pF3A and pF3K WG (BYDV) Flexi® Vectors are designed for enhanced expression in wheat germ extract.
Protocols and applications covering all aspects of eukaryotic, mammalian and bacterial cell-free expression.
Here we demonstrate the advantages of eukaryotic cell-free expression using ten difficult-to-express proteins and the TnT® T7 Insect Cell Extract and TnT® Quick Coupled Transcription/Translation Systems.