Promega Corporation

Magne™ Protein G and Magne™ Protein A Beads

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Magne™ Protein G and Magne™ Protein A Beads are magnetic affinity beads with high specificity and high capacity for purification of immunoglobulins from cell culture media, ascites and serum samples. These paramagnetic beads are composed of iron encapsulated in macroporous cellulose with low nonspecific binding. The magnetic beads use a novel attachment chemistry to immobilize recombinant Protein G or Protein A protein molecules in the same orientation on the surface of the bead. The oriented attachment is known to improve the functionality of immobilized proteins. These beads offer a...

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  • Prices valid for customers of Promega UK Ltd only
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Magne™ Protein G Beads, 20% Slurry

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Magne™ Protein G Beads, 20% Slurry

 Components

  • Magne™ Protein G Beads

    G747A1 x 1ml
1ml
- G7471 £ 73.00 Add to cart

Magne™ Protein G Beads, 20% Slurry

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Magne™ Protein G Beads, 20% Slurry

 Components

  • Magne™ Protein G Beads

    G747A5 x 1ml
5ml
(5 × 1ml)
- G7472 £ 294.00 Add to cart

Magne™ Protein G Beads, 20% Slurry

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Magne™ Protein G Beads, 20% Slurry

 Components

  • Magne™ Protein G Beads, 20% Slurry

    G747B1 x 50ml
50ml
- G7473 £ 2,194.00 Add to cart

Magne™ Protein A Beads, 20% Slurry

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Magne™ Protein A Beads, 20% Slurry

 Components

  • Magne™ Protein A Beads, 20% Slurry

    G878A1 x 1ml
1ml
- G8781 £ 73.00 Add to cart

Magne™ Protein A Beads, 20% Slurry

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Magne™ Protein A Beads, 20% Slurry

 Components

  • Magne™ Protein A Beads, 20% Slurry

    G878A5 x 1ml
5ml
(5 × 1ml)
- G8782 £ 294.00 Add to cart

Magne™ Protein A Beads, 20% Slurry

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Magne™ Protein A Beads, 20% Slurry

 Components

  • Magne™ Protein A Beads, 20% Slurry

    G878B1 x 50ml
50ml
- G8783 £ 2,194.00 Add to cart


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Storage Conditions

Store at 4°C. Do not freeze. Do not allow beads to dry during storage or use.

For product intended use please see Patents & Disclaimers tab.

Antibody purified from various sample types using the Magne™ Protein A and Magne™ Protein G Beads.Figure 1. Antibody purified from various sample types using the Magne™ Protein A and Magne™ Protein G Beads.

Antibody was purified from 50μl of cell culture media (mouse IgG1), mouse ascites (IgG2a) and goat serum with 50μl of Magne™ Protein A Beads (A) and Magne™ Protein G Beads (G) using published protocols (TM371). Samples were separated via SDS-PAGE by adding 1μl of starting material (SM) or 5μl of purified sample (A or G) and stained with Coomassie®-based stain.

Antibody was purified from 50μl of cell culture media (mouse IgG1), mouse ascites (IgG2a) and goat serum with 50μl of Magne™ Protein A Beads (A) and Magne™ Protein G Beads (G) using published protocols (TM371). Samples were separated via SDS-PAGE by adding 1μl of starting material (SM) or 5μl of purified sample (A or G) and stained with Coomassie®-based stain.

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10956CA_610pxFigure 2. Magne™ Protein G Beads-compatible magnetic separation stands for 0.5ml to 50ml tubes and 96-well plates (see Related Products).

Clockwise from top, PolyATtract® System Magnetic Separation Stand (15ml, 50ml tubes), MagneSphere® Magnetic Separation Stand (two-position), MagnaBot® Magnetic Separation Device and MagneSphere® Magnetic Separation Stand (twelve-position).

Clockwise from top, PolyATtract® System Magnetic Separation Stand (15ml, 50ml tubes), MagneSphere® Magnetic Separation Stand (two-position), MagnaBot® Magnetic Separation Device and MagneSphere® Magnetic Separation Stand (twelve-position).

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Schematic of antibody purification using Magne™ Protein A Beads or Magne™ Protein G Beads.Figure 3. Schematic of antibody purification using Magne™ Protein A Beads or Magne™ Protein G Beads.

Inset: Magne™ Protein A Beads captured on a magnet.

Relative binding affinities of Protein A and Protein G for different antibody species and isotypes.Table 1. Relative binding affinities of Protein A and Protein G for different antibody species and isotypes.

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Use Restrictions

G7471, G7472, G7473, G8781, G8782, G8783 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G7471, G7472, G7473, G8781, G8782, G8783 Patent Pending.

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