Promega Corporation

HaloCHIP™ System

The HaloCHIP™ System is a novel method designed for the covalent capture of intracellular protein:DNA complexes without the use of antibodies and offers an efficient and robust alternative to the standard chromatin immunoprecipitation (ChIP) method. Proteins of interest are expressed in cells as HaloTag® fusion proteins, crosslinked to DNA with formaldehyde and then captured on HaloLink™ Resin, which forms a highly specific, covalent interaction with the HaloTag® portion of the fusion protein. Stringent washing removes nonspecific proteins and DNA, and heating reverses the crosslinks between the DN...

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HaloCHIP™ System

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HaloCHIP™ System

 Components

  • HaloLink™ Resin

    G191A1 x 0.5ml
  • Mammalian Lysis Buffer

    G938A1 x 40ml
  • High Salt Wash Buffer

    G939A1 x 25ml
  • Reversal Buffer

    G940A1 x 8ml
  • HaloCHIP™ Blocking Ligand

    G54911 x 30μl
  • Nuclease-Free Water

    P11951 x 150ml
  • TE Buffer, 1X, Molecular Biology Grade

    V62311 x 100ml
20 reactions
- G9410 £ 287.00 Add to cart


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Storage Conditions

The TE Buffer (pH 8.0), Reversal Buffer and Nuclease-Free Water may be stored at room temperature. Store the HaloLink™ Resin, Mammalian Lysis Buffer and High Salt Wash Buffer at 4°C. Store the HaloCHIP™ Blocking Ligand at –20°C.

For product intended use please see Patents & Disclaimers tab.

Representation of the HaloCHIP System.Figure 1. Representation of the HaloCHIP™ System.

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Use Restrictions

G9410 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G9410 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag® ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G9410 U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.

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