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Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum.
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For more information, see the Protein Expression chapter of the Protocols & Applications Guide.
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Wheat Germ Extract
Amino Acid Mixture Minus Cysteine
Luciferase Control RNA
Amino Acid Mixture Minus Leucine
Amino Acid Mixture Minus Methionine
Store at –70°C or below. Avoid freeze-thaw cycles.
For product intended use please see Patents & Disclaimers tab.
L4380 For Research Use Only. Not for Use in Diagnostic Procedures.