Choose from an extensive range of eukaryotic cell-free protein expression systems. Kits optimized for in vitro transcription and translation of plasmid DNA, RNA or PCR DNA are available.
TnT® SP6 High-Yield Wheat Germ System
Express up to 100µg of soluble, functional, full-length protein in as little as 60 minutes directly from plasmid DNA.
TnT® Quick Systems
Add plasmid DNA directly to the TnT® Master Mix to express protein quickly in this eukaryotic-based system. No purification required after expression—use directly for protein interaction experiments.
TnT® T7 Insect Cell Extract
A convenient alternative to resource and time constrained cell-based baculovirus expression.
TnT® T7 Quick for PCR DNA
Convenient protein expression directly from PCR templates. No post-amplification clean-up required.
This article describes cell-free expression and capture of histone deacetylase (HDAC) proteins using the TnT® T7 Insect Cell Extract System, HaloTag® labeling and Maxwell® 16 isolation technologies.