T3 RNA Polymerase

T3 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T3 DNA or DNA cloned downstream from a T3 promoter can serve as a template for T3 RNA Polymerase-directed RNA synthesis.

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T3 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T3 DNA or DNA cloned downstream from a T3 promoter can serve as a template for T3 RNA Polymerase-directed RNA synthesis.

Features - Benefits

• Specific: T3 RNA Polymerase exhibits extremely high affinity and specificity for T3 promoter sequences.
• Highly Pure: T3 RNA Polymerase is >90% pure as determined by SDS polyacrylamide gel electrophoresis. Free of detectable levels of contaminating RNase and DNase activity (<1% release).
• Flexible: Will incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates.
• Provided with 5X Reaction Buffer: Provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl₂, 10mM spermidine, 50mM NaCl.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Generation of templates for in vitro translation.
• Generation of probes for nucleic acid hybridizations.
• Generation of RNA processing substrates.
• Generation of antisense RNA.

References

1. Butler, E.T. and Chamberlin, M.J. (1982) J. Biol. Chem. 257, 5772–8.
2. Melton, D.A. et al. (1984) Nucl. Acids Res. 12, 7035–56.

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