Promega Corporation

Cloning Tools and Competent Cells

Start collecting with Promega restriction enzymesTry our cheeky, little promotion!Less than half the price of Amersham ECL!FMG-skip-the-cloning

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Subcloning tools 9505CA
New Single-Use Competent Cells

The Single-Use Cells are 50µl aliquots of chemically competent cells in single-use tubes. Transformation is performed directly in the provided tube, saving you time and effort and minimizing loss of efficiency due to repeated freeze-thaw cycles.

Featured Products

Shine on you crazy Diamond Dye....at half price now!

Diamond™ Nucleic Acid Dye provides a robust method for staining many gel types, along with the convenience of room temperature stability. It's more sensitive than ethidium bromide, so you use less sample and ladders for visualisation, saving you money with every gel you run.

Comparable in performance to SYBR® Gold, it is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system.

Diamond™ Nucleic Acid Dye does not require prewashing or destaining of gels.

Try Diamond Dye now for half price, that's just £27.50 for 500μl !

To claim your 50% discount on Diamond Dye please quote Diamond 50 when you order H1181.

Offer ends June 30th 2013.

Single Step KRX Competent Cells

Efficient transformation and tightly controlled protein expression. Ideal for rapidly generating new clones for recombinant protein expression.

Single-Use Pro 5-alpha Competent Cells

High-efficiency competent cells (109cfu/µg), ideal for the blue/white screening of recombinant clones. Pro 5-alpha can be used for the efficient transformation of unmethylated DNA from PCR, cDNA and many other sources.

pGEM® 3Zf+/- Vectors

Multi-purpose, blue/white screening vector with many unique restriction sites. Use as a standard cloning vector, for in vitro transcription, or for production of circular ssDNA.

T4 DNA Ligase

Blue/White cloning qualified. Use to join two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either sticky- or blunt-end configuration.

Featured Resources

Tools_fr BioMath Calculators

The easy way to calculate melting temperature, molarity, dilution, DNA and protein concentration.

teachtrain_fr Unit 6: Basics of Molecular Cloning (Blue/White Selection)

This teaching unit covers the basics of cloning and blue/white selection. Includes math-intensive lab

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