Promega Corporation

AMP-Glo™ Assay

The AMP-Glo™ Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP as a reaction product. This versatile system can measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. The AMP-Glo™ Assay is designed to quantitatively monitor the concentration of AMP in a biochemical reaction in a wide range of plate formats, including high-throughput formats. The stable luminescent signal of the assay eliminates the need...

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  • Prices valid for customers of Promega UK Ltd only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart
AMP-Glo™ Assay
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AMP-Glo™ Assay  Components

  • AMP-Glo™ Reagent I

    V503A1 x 5ml
  • AMP-Glo™ Reagent II

    V504A1 x 100μl
  • Kinase-Glo® One Solution

    V505A1 x 10ml
  • AMP, 10mM

    V506A1 x 100μl
  • Ultra Pure ATP, 10mM

    V915A1 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 assays
- V5011 £ 407.00 Add to cart
AMP-Glo™ Assay
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AMP-Glo™ Assay  Components

  • AMP-Glo™ Reagent I

    V503B1 x 50ml
  • AMP-Glo™ Reagent II

    V504B1 x 1ml
  • Kinase-Glo® One Solution

    V505B1 x 100ml
  • AMP, 10mM

    V506B1 x 1ml
  • Ultra Pure ATP, 10mM

    V915B1 x 5ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10,000 assays
- V5012 £ 2,421.00 Add to cart
AMP-Glo™ Assay
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AMP-Glo™ Assay  Components

  • AMP-Glo™ Reagent I

    V503C1 x 250ml
  • AMP-Glo™ Reagent II

    V504C1 x 5ml
  • Kinase-Glo® One Solution

    V505C1 x 500ml
  • AMP, 10mM

    V506C1 x 5ml
  • Ultra Pure ATP, 10mM

    V915B5 x 5ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50,000 assays
- V5013 £ 6,129.00 Add to cart


Storage Conditions

Store the system at –30 to –10°C. Before use, thaw all components completely at room temperature, except for the AMP-Glo™ Reagent II, which should be kept on ice after thawing. Once thawed, mix all components thoroughly before use. Once prepared, the Kinase-Glo® One Solution should be dispensed into aliquots and stored at –20°C. See the product label for expiration date.

For product intended use please see Patents & Disclaimers tab.

AMP-Glo™ Assay principle.Figure 1. AMP-Glo™ Assay principle.

The AMP-Glo™ Assay can be used to detect activity of enzymes that catalyze any reaction that produces AMP as a reaction product, including enzymes that do not use ATP as a substrate (e.g., cAMP-specific PDE, poly(A) deadenylases, ribonucleases, bacterial DNA ligase) as well as enzymes that use ATP as a substrate (e.g., ubiquitin ligase, aminoacyl tRNA synthetase, eukaryotic DNA ligase, succinyl CoA synthetase). After completing the enzymatic reaction, adding AMP-Glo™ Reagent I terminates the reaction, removes any remaining ATP, and converts AMP to ADP. Adding AMP Detection Solution drives the conversion of ADP to ATP and the detection of ATP through the luciferase reaction. The amount of AMP produced by the reaction is proportional to the light measured and can be extrapolated using a standard curve.

The AMP-Glo™ Assay can be used to detect activity of enzymes that catalyze any reaction that produces AMP as a reaction product, including enzymes that do not use ATP as a substrate (e.g., cAMP-specific PDE, poly(A) deadenylases, ribonucleases, bacterial DNA ligase) as well as enzymes that use ATP as a substrate (e.g., ubiquitin ligase, aminoacyl tRNA synthetase, eukaryotic DNA ligase, succinyl CoA synthetase). After completing the enzymatic reaction, adding AMP-Glo™ Reagent I terminates the reaction, removes any remaining ATP, and converts AMP to ADP. Adding AMP Detection Solution drives the conversion of ADP to ATP and the detection of ATP through the luciferase reaction. The amount of AMP produced by the reaction is proportional to the light measured and can be extrapolated using a standard curve.

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Use Restrictions

V5011, V5012, V5013 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V5011, V5012, V5013 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

V5011, V5012, V5013 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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