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BacTiter-Glo™ Microbial Cell Viability Assay

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The BacTiter-Glo™ Microbial Cell Viability Assay is a homogeneous method for determining the number of viable microbial cells in culture based on quantitation of the ATP present. ATP is an indicator of metabolically active cells. The homogeneous assay procedure involves adding a single reagent (BacTiter-Glo™ Reagent) directly to bacterial cells cultured in medium and measuring luminescence. The homogeneous format reduces pipetting errors that may be introduced during the multiple steps required by other methods of ATP measurement. The formulation of the reagent supports bacterial cell lysis and generation of a lum...

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  • Prices valid for customers of Promega UK Ltd only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

BacTiter-Glo™ Microbial Cell Viability Assay

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BacTiter-Glo™ Microbial Cell Viability Assay

 Components

  • BacTiter-Glo™ Buffer

    G818A1 x 10ml
  • BacTiter-Glo™ Substrate

    G819A1 x 10ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10ml
- G8230 £ 54.00 Add to cart

BacTiter-Glo™ Microbial Cell Viability Assay

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BacTiter-Glo™ Microbial Cell Viability Assay

 Components

  • BacTiter-Glo™ Buffer

    G818A10 x 10ml
  • BacTiter-Glo™ Substrate

    G819A10 x 10ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10 × 10ml
- G8231 £ 322.00 Add to cart

BacTiter-Glo™ Microbial Cell Viability Assay

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BacTiter-Glo™ Microbial Cell Viability Assay

 Components

  • BacTiter-Glo™ Buffer

    G818B1 x 100ml
  • BacTiter-Glo™ Substrate

    G819B1 x 100ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100ml
- G8232 £ 286.00 Add to cart

BacTiter-Glo™ Microbial Cell Viability Assay

Close Window

BacTiter-Glo™ Microbial Cell Viability Assay

 Components

  • BacTiter-Glo™ Buffer

    G818B10 x 100ml
  • BacTiter-Glo™ Substrate

    G819B10 x 100ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10 × 100ml
- G8233 £ 2,148.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

rATP, 10mM

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rATP, 10mM

 Components

  • rATP

    P113B1 x 0.5ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


0.5ml
- P1132 £ 39.00 Add to cart

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Storage Conditions

For long-term storage, the lyophilized BacTiter-Glo™ Substrate and BacTiter-Glo™ Buffer should be stored at –20°C.

For product intended use please see Patents & Disclaimers tab.

Evaluating bacterial growth using the BacTiter-Glo Assay.Figure 1. Evaluating bacterial growth using the BacTiter-Glo™ Assay.

E. coli ATCC 25922 strain was grown in Mueller Hinton II (MH II) Broth at 37°C overnight. The overnight culture was diluted 1:106 in 50ml of fresh MH II Broth and incubated at 37°C with shaking at 250rpm. Samples were taken at various time points, and the BacTiter-Glo™ Assay was performed. Luminescence was recorded on a GloMax® 96 Microplate Luminometer. Optical density was measured at 600nm (O.D.600) using a Beckman DU650 spectrophotometer. Diluted samples were used when readings of RLU and O.D. exceeded 108 and 1, respectively.

Overview of the BacTiter-Glo Microbial Cell Viability Assay protocol.Figure 2. Overview of the BacTiter-Glo™ Microbial Cell Viability Assay protocol.

Use Restrictions

G8230, G8231, G8232, G8233 For Research Use Only. Not for Use in Diagnostic Procedures.
P1132 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

G8230, G8231, G8232, G8233 U.S. Pat. No. 7,422,868 and other patents and patents pending.

G8230, G8231, G8232, G8233 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G8230, G8231, G8232, G8233 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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